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Figure 1 | Retrovirology

Figure 1

From: Design of a trans protease lentiviral packaging system that produces high titer virus

Figure 1

Schematic of the components involved in the 5, 6, and 7 plasmid systems. (A) Diagram of the 4 plasmids used in common for all three packaging systems for the production of virus, followed by a brief description of the packaging plasmids used for each of the corresponding systems (more detail is shown in Fig. 2). (B) Schematic depicting the assembly site of the viral proteins as it takes place in the 5 plasmid system, here the Gag and Gag-Pol precursor proteins are targeted to the cell membrane through the membrane-binding domain located at the N-terminus of MAp17, and the assembly sites of the 6 and 7 plasmid systems where the Gag proteins are targeted to cell membrane by MAp17, and the Pol proteins (PR and RT/IN) are targeted through tethering of Vpr to P6.

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