Figure 2
From: Differential resistance to cell entry by porcine endogenous retrovirus subgroup A in rodent species
Identification of critical amino acid residues for PERV-A infection. HA-tagged chimeric receptors (H2M a-f) between huPAR-2 (white bars) and muPAR (black bars) as well as huPAR-1 (grey bar) and the mutant H1M g were introduced into QT6 cells by MLV-based retroviral vectors. 50–70% of the QT6 cell population showed PAR expression as confirmed by anti-HA staining. These cultures were infected with EGFP(PERV-A). Cells were harvested 48 hours later and PERV-A infection was measured by flow cytometry as percentage of EGFP-positive cells. Arrows indicate infection below detectable levels. Results are expressed as average ± SEM from three independent experiments.