Figure 1

PERV-A receptor function of HuPARs and their rodent homologues. A. The different cell lines were transduced with the same amount of retroviral vector encoding the HA-tagged receptor genes. Transduced cells were then infected with EGFP(PERV-A). 48 hours post-infection cells were analysed by flow cytometry and the efficiency of infection was determined as percentage of EGFP positive cells. The histograms represent the average ± SEM from three independent experiments. The arrows indicate an infection below detectable levels. B. NRK, HSN and XC rat cells were transduced with a retroviral vector encoding the ratPAR gene. Two independent transductions were performed on NRK and HSN cells. The RNA from transduced and untransduced rat cells were extracted. The amount of ratPAR was determined by real time RT-PCR and normalised to equalised copies of 18S rRNA. The results were correlated with the efficiency of EGFP(PERV-A) infection. All the samples were run in duplicate and the experiment repeated at least two times.