Figure 7
Effect of Tax on HBZ-mediated activation of the hTERT promoter. (A) The luciferase reporter plasmid, pGL3-378 (100 ng) was cotransfected with expression plasmids for HBZ (200 ng), JunD (200 ng) and Tax (10 ng), as indicated. Luciferase activity, normalized to tk-luc activity, is presented relative to that of cells transfected with the reporter plasmid alone. Results represent triplicate samples, from three different experiments. (B) Western blot analysis of HBZ, Tax and JunD protein levels in whole cell lysates of HeLa samples. The membrane was successively probed with a rabbit polyclonal anti-HBZ, anti-Tax sera, mouse monoclonal anti-Flag and rabbit anti-actin antibodies.