Figure 6

Functional interactions between HBZ, JunD and Sp1. (A) HeLa cells were cotransfected with the reporter construct 5XGal4-Luc (200 ng) consisting of five tandem Gal4-binding sites in the absence (-) or presence (100 ng) of expression vectors for Gal4-Sp1B (containing the B domain of Sp1), and/or HBZ and JunD. Luciferase activity was normalized to tk-luc activity. Results represent duplicate samples from three different experiments. (B) Western blot analysis of Sp1, HBZ and JunD protein levels in whole cell lysates of HeLa samples. The membrane was successively probed with rabbit polyclonal anti-Sp1, anti-HBZ sera, and mouse monoclonal anti-Flag and anti-actin antibodies.