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Figure 2 | Retrovirology

Figure 2

From: HTLV-1 HBZ cooperates with JunD to enhance transcription of the human telomerase reverse transcriptase gene (hTERT)

Figure 2

Deletion of the activation domain and leucine-zipper region of HBZ significantly reduces hTERT promoter activity. The luciferase reporter plasmid, pGL3-378 (100 ng) was cotransfected with expression plasmids (200 ng) for JunD, HBZ or HBZΔAD or HBZΔbZIP, or HBZΔADΔZip as indicated. Luciferase activity was normalized to tk-luc activity and is presented relative to that of cells transfected with the reporter plasmid alone. The values are those obtained in duplicate from five experiments. Lower panel: western blot analysis of HBZ and JunD protein levels in whole cell lysates of HeLa samples. The membrane was successively probed with monoclonal anti-c-myc, anti-Flag and anti-actin antibodies.

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