Mutations in the HIV-2 packaging signal affect replication of HIV-2 in T-cells. 1 × 106 Jurkat T-cells were infected with an amount of virus equivalent to 1 × 107 cpm as measured by RT activity assay. Replication was assessed by measure of the RT activity every 4 days. Cells were passaged 1/2 every 8 days but no new cells or viruses were added at any point during the course of the infection. Data from two independent experiments are shown. Error bars represent the SD.