Asn 362 in gp120 contributes to enhanced fusogenicity by CCR5-restricted HIV-1 envelope glycoprotein variants from patients with AIDS

Table 1 Characteristics of primary R5 viruses and Env clones

Virus^a	Description^b	Env clone^c	Coreceptor usage^d
			CCR5	CXCR4
Cross-sectional viruses				-
NB23	PA-R5	NB23-C1	+++	-
		NB23-C2	+++	-
		NB23-C3	+++	-
NB24	PA-R5	NB24-C1	++	-
		NB24-C2	+	-
		NB24-C3	+	-
		NB24-C4	+	-
NB25	PA-R5	NB25-C1	+	-
		NB25-C2	+	-
		NB25-C3	+++	-
NB27	PA-R5	NB27-C1	+	-
		NB27-C2	++	-
		NB27-C3	+++	-
NB2	A-R5	NB2-C1	+	-
		NB2-C2	+	-
		NB2-C3	++	-
		NB2-C4	++	-
NB6	A-R5	NB6-C1	+++	-
		NB6-C2	+++	-
		NB6-C3	+++	-
		NB6-C4	+++	-
NB7	A-R5	NB7-C1	++	-
		NB7-C2	++	-
		NB7-C3	++	-
		NB7-C4	++	-
NB8	A-R5	NB8-C1	+++	-
		NB8-C2	+++	-
		NB8-C3	+++	-
		NB8-C4	+++	-
Longitudinal viruses
IK1-PA	PA-R5	IK1-PA-C1	++	-
		IK1-PA-C2	+	-
		IK1-PA-C3	++	-
		IK1-PA-C4	+	-
IK1-A	A-R5	IK1-A-C1	++	-
		IK1-A-C2	+++	-
		IK1-A-C3	++	-
		IK1-A-C4	++	-
Controls
		ΔKS Env	-	-
		HXB2 Env	-	+++
		ADA Env	+++	-
		89.6 Env	+++	+++

^aThe phenotypes of the primary R5 HIV-1 isolates, and clinical characteristics of the subjects from whom they were isolated have been described in detail previously [11, 13, 34, 36, 42].
^bPA-R5, pre-AIDS R5 HIV-1 isolate; A-R5, AIDS R5 HIV-1 isolate.
^cFunctional Env clones were identified by infection of JC53 cells or Cf2-CD4/CCR5/CXCR4 cells with Env-pseudotyped GFP reporter viruses and by fusion assays, as described in the Methods (data not shown).
^dCoreceptor usage of functional Envs was determined by infection of Cf2-CD4/CCR5 and Cf2-CD4/CXCR4 cell lines with Env-pseudotyped GFP-reporter viruses, as described in the Methods. GFP positive cells were counted manually by fluorescence microscopy and scored as – (no GFP positive cells), +/- (1 to 5% GFP positive cells), + (5 to 10% GFP positive cells), ++ (10 to 30% GFP positive cells), or +++ (>30% GFP positive cells).

ISSN: 1742-4690