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Table 1 Characteristics of primary R5 viruses and Env clones

From: Asn 362 in gp120 contributes to enhanced fusogenicity by CCR5-restricted HIV-1 envelope glycoprotein variants from patients with AIDS

Virusa Descriptionb Env clonec Coreceptor usaged
    CCR5 CXCR4
Cross-sectional viruses     -
NB23 PA-R5 NB23-C1 +++ -
   NB23-C2 +++ -
   NB23-C3 +++ -
NB24 PA-R5 NB24-C1 ++ -
   NB24-C2 + -
   NB24-C3 + -
   NB24-C4 + -
NB25 PA-R5 NB25-C1 + -
   NB25-C2 + -
   NB25-C3 +++ -
NB27 PA-R5 NB27-C1 + -
   NB27-C2 ++ -
   NB27-C3 +++ -
NB2 A-R5 NB2-C1 + -
   NB2-C2 + -
   NB2-C3 ++ -
   NB2-C4 ++ -
NB6 A-R5 NB6-C1 +++ -
   NB6-C2 +++ -
   NB6-C3 +++ -
   NB6-C4 +++ -
NB7 A-R5 NB7-C1 ++ -
   NB7-C2 ++ -
   NB7-C3 ++ -
   NB7-C4 ++ -
NB8 A-R5 NB8-C1 +++ -
   NB8-C2 +++ -
   NB8-C3 +++ -
   NB8-C4 +++ -
Longitudinal viruses     
IK1-PA PA-R5 IK1-PA-C1 ++ -
   IK1-PA-C2 + -
   IK1-PA-C3 ++ -
   IK1-PA-C4 + -
IK1-A A-R5 IK1-A-C1 ++ -
   IK1-A-C2 +++ -
   IK1-A-C3 ++ -
   IK1-A-C4 ++ -
Controls     
   ΔKS Env - -
   HXB2 Env - +++
   ADA Env +++ -
   89.6 Env +++ +++
  1. aThe phenotypes of the primary R5 HIV-1 isolates, and clinical characteristics of the subjects from whom they were isolated have been described in detail previously [11, 13, 34, 36, 42].
  2. bPA-R5, pre-AIDS R5 HIV-1 isolate; A-R5, AIDS R5 HIV-1 isolate.
  3. cFunctional Env clones were identified by infection of JC53 cells or Cf2-CD4/CCR5/CXCR4 cells with Env-pseudotyped GFP reporter viruses and by fusion assays, as described in the Methods (data not shown).
  4. dCoreceptor usage of functional Envs was determined by infection of Cf2-CD4/CCR5 and Cf2-CD4/CXCR4 cell lines with Env-pseudotyped GFP-reporter viruses, as described in the Methods. GFP positive cells were counted manually by fluorescence microscopy and scored as – (no GFP positive cells), +/- (1 to 5% GFP positive cells), + (5 to 10% GFP positive cells), ++ (10 to 30% GFP positive cells), or +++ (>30% GFP positive cells).