Intracellular Distribution of Peptide 2. H9 and MT2 cells were treated with 50 μM Peptide 2-FITC and after 5 minutes of incubation, the cells were fixed, mounted with DAPI-containing media and fluorescence microscopy was performed with filters for DAPI and FITC as described in Methods. Longer durations of treatment were also evaluated in a similar manner. The images were manually overlaid to superimpose the image of the nucleus with each image of Peptide 2-FITC distribution in the cell. Cells from two different regions of the H9 and MT2 plates are shown.