Figure 1
From: Isolated HIV-1 core is active for reverse transcription
Analysis of core fractions. (A) Endogenous reverse transcriptase activity: strong-stop (squares), first-strand transfer (diamonds) and full-length targets (triangles) are shown. (B) p24 ELISA on fractions; inset shows the density of fractions calculated from weight (fractions 3–9 only are shown). Viral proteins were detected in HIV-1NL4.3 equilibrium gradient fractions 1–9 by western analysis using (C) anti-HIV-1 polyclonal antibody, (D) colorimetric reverse transcriptase ELISA using homoploymeric template (fractions 5–9 only are shown), and (E) anti-gp41 antibody. (F) Negative staining transmission electron microscopy of dense fractions showing four representative core-like structures. 100,000× magnification; bar indicates 50 nm. Please note, the fractions shown in A and B are from a separate preparation to those in C-E and hence fraction numbers do not directly correspond [see Additional file 1 for complete methods].