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Figure 4 | Retrovirology

Figure 4

From: Requirements for the selective degradation of CD4 receptor molecules by the human immunodeficiency virus type 1 Vpu protein in the endoplasmic reticulum

Figure 4

Effect of Vpu on CD4 KRcyto poly-ubiquitination. A. HEK 293T cells were mock-transfected or co-transfected with 1 μg of pHIV CD4 wt or pHIV CD4 KRcyto, 10 μg of provirus encoding Vpu- (HxBH10-vpu-) or Vpu+ (HxBH10-vpu+) and 25 μg of the TDN mutant of Ub his(6)/c-myc-Ub K48/R. Transfected cells were not treated with BFA prior to lysis. Samples were then treated as described in the materials and methods. B. Quantitative analysis of the relative levels of ubiquitinated CD4 conjugates for CD4 wt and CD4 KRcyto in two independent experiments. (asterisk) represents the area of the autoradiogram that was used for the quantitation of CD4-Ub conjugates. Relative levels of ubiquitinated CD4 conjugates were determined as described in Fig. 2B.

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