Figure 8
From: Rapid spread of mouse mammary tumor virus in cultured human breast cells
Virus infecting human cells do not contain env gene derived from endogenous retroviruses. (A) The PCR products encompassing complete env coding region amplified from the cell lysates of GR cells (lane 1) and third-round infected Hs578T cells (lane 2) were submitted to digestion with NdeI. As a digestion control, PCR product obtained by amplification of MTV-8 env sequences using pGR16 plasmid as a template, was digested with the same restriction enzyme (lane 3); M, 1 kb marker; black arrow indicates undigested product; open arrows denote fragments resulting from NdeI digestion. (B) Schematic drawing showing NdeI site in the env gene of the Mtv-17 and Mtv-8 viruses and the length of the respective restriction fragments.