Figure 6
From: Detection, characterization and regulation of antisense transcripts in HIV-1
Generation of a proviral DNA construct expressing the antisense directed luciferase gene expression. (A) 293T cells were transfected with 600 ng pNL4.3AsLucR-E- and lysed 48 h post-transfection. CTL represents untransfected cells. (B-C) Jurkat cells were transfected with 15 μg pNL4.3AsLucR-E- (B) or pNL4.3ΔBstAsLucR-E- (C) and stimulated with different activators for 8 h. or left untreated. Lysed cells were then analysed for luciferase activity. Results show the mean luciferase activity values of three measured samples ± S.D (A-B) or fold induction compared to the unstimulated control (C).