Figure 5
From: Detection, characterization and regulation of antisense transcripts in HIV-1
Identification of a new functional polyadenylation site for HIV-1 antisense transcript. (A) Total RNA from 293T cells transfected with pNL4.3ΔNarI was used for 3'RACE analysis. CTL represents PCR amplification conducted in the absence of cDNA and RNA samples. The amplified product was cloned and sequenced. M = 100 bp marker (the asterisk indicates the 600 bp band). (B) Position of the newly identified antisense polyA addition site (pA indicated with arrow) in the HIV-1 genome. (C) Sequence alignment of 8 HIV-1 genome showing conservation for the consensus polyA signal and of a GT-rich consensus sequence. Position of the polyA addition site is indicated by an arrow.