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Figure 4 | Retrovirology

Figure 4

From: Detection, characterization and regulation of antisense transcripts in HIV-1

Figure 4

Identification of transcription initiation sites for the antisense transcript. Total RNA from 293T cells transfected with the pNL4ΔNar1 construct was used to amplify 5' cDNA ends using the 5' RACE approach. Numerous transcription initiation sites were identified upon sequencing of multiple PCR-amplified signals and are part of a region presented in the enlarged segment encompassing the LTR, and both nef and env gene regions. Nucleotide numbering corresponds to the sense strand.

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