Fate-of-capsid assay. MDTF and D17 cells expressing TRIMCyp, and control cells were infected with HIV-1 VLPs as in Fig. 5. The cells expressing TRIMCyp were infected in the presence or not of cyclosporine (5 μM). CA was detected in post-sedimentation pellets and supernatants. Pellet CA was quantitated as in Fig. 5.