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Figure 3 | Retrovirology

Figure 3

From: Both TRIM5α and TRIMCyp have only weak antiviral activity in canine D17 cells

Figure 3

Restriction of HIV-1. A, MDTF or D17 cells expressing TRIM5αrh, TRIMCyp, or control cells were infected with multiple dilutions of HIV-1GFP, an HIV-1 vector expressing GFP. The percentage of infected cells was determined by flow cytometry. B, MDTF, HeLa or D17 cells expressing TRIM5αrh or TRIMCyp, and control cells were infected with HIV-1GFP. The virus dose used in each cell type was first adjusted so that 5–10% of control cells would be infected and the same volume of virus preparation was then used to infect the various TRIM5-expressing cell lines from that cell type. The percentage of infected cells was determined 2 days later by flow cytometry, and results are shown as % of the values obtained for the control cells. The experiment was carried out in triplicates, and standard deviations are shown.

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