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Figure 6 | Retrovirology

Figure 6

From: Unintegrated HIV-1 provides an inducible and functional reservoir in untreated and highly active antiretroviral therapy-treated patients

Figure 6

Characterization of preintegrated HIV-1 DNA using Alu -LTR real-time PCR. The level of integrated HIV-1 DNA copies was assessed in CD4+ T lymphocytes from the in vitro model of infection and from four patients (nos. 4, 12, 13 and 14) using Alu-LTR real-time PCR experiments. CD4+ T cells that were directly stimulated, preincubated 1 and 2 days before polyclonal activation, and directly stimulated and cultured with L-731,988 were recovered from ELISpot assays and tested in PCR experiments.

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