Skip to main content
Figure 1 | Retrovirology

Figure 1

From: Unintegrated HIV-1 provides an inducible and functional reservoir in untreated and highly active antiretroviral therapy-treated patients

Figure 1

Experimental protocol and culture conditions. A. In order to study the mobilization of the functional preintegration reservoir, resting CD4+ T cells were activated and cultured with the HIV-1 integrase inhibitor L-731,988 at the final concentration of 40 μM. B. To assess the correlation between the unintegrated HIV-1 DNA decay in vitro and the decline of rescuable viral production, infected resting CD4+T cells were preincubated 1 or 2 days before polyclonal stimulation. In both cases, in order to prevent infection of others cells by de novo-synthesized HIV-1, 1 μg/ml of the viral entry inhibitor T20 was also added in culture medium.

Back to article page