Viral replication of C33A produced viruses in the presence of CQ. A) JR-CSF (R5) replication, B) 299.10 (R5X4) replication and C) LAI (X4) replication. All three viruses were produced by transfection of C33A cells pre-treated with 100 μM CQ or in its absence as a control. The replication capacity of the produced viruses were determined on CD4+ T-lymphocytes in the absence of CQ. CA-p24 at 1 ng/ml was used as viral input with the CA-p24 concentration determined during the course of the infection. Standard deviations are depicted in all panels. All replications were performed in triplicate.