Figure 6
From: Basal shuttle of NF-κB/IκBα in resting T lymphocytes regulates HIV-1 LTR dependent expression
HIV replication in resting or activated CD 4 + T cells transfected with an infectious molecular HIV-1 clone. Highly purified CD4+ CD25 - CD69 - DR- T cells were transfected with the NL4.3 infectious molecular HIV-1 clone together with CMV-IκBα or pcDNA3.1 as negative control, and then activated with anti-CD3 and IL-2, PHA and IL-2, or maintained in the absence of activation. Viral replication was determined by quantification of HIV p24-gag antigen in culture supernatants (a) after 5 days of transfection or (b) after 7 days of transfection. Numbers on the top of the bars represent fold HIV-replication relative to unstimulated T cells transfected with pcDNA3.1. Differences in p24-gag production were significant for resting and anti-CD3-activated T cells (p < 0.05) and a trend towards statistical significance was found in PHA-activated T cells (p = 0.081).