|
Origin of cells for virus isolation
|
Isolatea
|
NP-2/CD4/CCR5 syncytiab
|
NP-2/CCR5 syncytia
|
NP-2/CCR5+PBMC virus production c RT (pg/ml)
|
|---|
| |
Monkey
|
Time PI (months)
| | | | |
|---|
|
mPBMC
|
D24
|
0.5
| |
++++
|
+++
|
>1000
|
| | |
3
| |
++++
|
+
|
803
|
| | |
10
|
§
|
+++
|
-
|
560
|
| |
C73
|
5
|
§
|
++++
|
++
|
>1000
|
| | |
7
|
§
|
++++
|
++
|
649
|
| | |
18
| |
++++
|
+
|
450
|
| |
C68
|
0.5
|
#
|
++
|
-
|
526
|
| | |
30
|
#
|
++
|
-
|
674
|
| | |
53
|
#
|
++
|
-
|
78
|
| |
B173
|
0.5
| |
++++
|
+++
|
998
|
| | |
39
| |
++++
|
+
|
827
|
|
hPBMC
|
D24
|
0.5
| |
+++
|
-
|
>1000
|
| | |
3
| |
++
|
-
|
713
|
| | |
12
|
§
|
-
|
-
|
<50
|
| |
C73
|
0.5
|
§
|
++
|
-
|
573
|
| | |
3
|
§
|
++
|
-
|
161
|
| | |
18
| |
++++
|
-
|
>1000
|
| |
C68
|
0.5
| |
++++
|
-
|
<50
|
| | |
3
|
§
|
++++
|
-
|
>1000
|
| | |
30
| |
++++
|
-
|
741
|
| | |
53
| |
++++
|
-
|
762
|
| |
B173
|
0.5
| |
++++
|
+/-
|
>1000
|
| | |
90
|
§
|
++
|
-
|
>1000
|
| | |
39
| |
+
|
-
|
85
|
- a Cells were infected with virus stocks containing 2.7–3.5 log10 pg RT/well except for indicated (#) isolates that were infected with 1.9–2.3 log10 pg RT/well. §Isolates that could not be obtained on corresponding time-points when isolating viruses on mPBMC and hPBMC, respectively. PI, time for virus isolation post infection.
- b Induction of syncytia was observed in light microscope 5 and 7 days after infection. -, no syncytia; +, 10–20 syncytia per well; ++, syncytia covering 20–50% of the wells; +++, syncytia covering 50–90% of the wells; ++++, syncytia covering >90% of the wells
- c Virus production was measured six days after start of cocultures with human PBMC (hPBMC). Values are means of two independent infections in duplicate wells. Supernatant culture fluids were collected at day 7 and production of RT was analyzed. Supernatants were undiluted in the RT assay and therefore values above 1000 pg RT/ml cannot be separated. Cut-off detection level was 50 pg RT/ml
