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Table 1 Comparison of the capacity to infect NP-2 cells by isolates on mPBMC or hPBMC.

From: CD4-independent use of the CCR5 receptor by sequential primary SIVsm isolates

Origin of cells for virus isolation Isolatea NP-2/CD4/CCR5 syncytiab NP-2/CCR5 syncytia NP-2/CCR5+PBMC virus production c RT (pg/ml)
  Monkey Time PI (months)     
mPBMC D24 0.5   ++++ +++ >1000
   3   ++++ + 803
   10 § +++ - 560
  C73 5 § ++++ ++ >1000
   7 § ++++ ++ 649
   18   ++++ + 450
  C68 0.5 # ++ - 526
   30 # ++ - 674
   53 # ++ - 78
  B173 0.5   ++++ +++ 998
   39   ++++ + 827
hPBMC D24 0.5   +++ - >1000
   3   ++ - 713
   12 § - - <50
  C73 0.5 § ++ - 573
   3 § ++ - 161
   18   ++++ - >1000
  C68 0.5   ++++ - <50
   3 § ++++ - >1000
   30   ++++ - 741
   53   ++++ - 762
  B173 0.5   ++++ +/- >1000
   90 § ++ - >1000
   39   + - 85
  1. a Cells were infected with virus stocks containing 2.7–3.5 log10 pg RT/well except for indicated (#) isolates that were infected with 1.9–2.3 log10 pg RT/well. §Isolates that could not be obtained on corresponding time-points when isolating viruses on mPBMC and hPBMC, respectively. PI, time for virus isolation post infection.
  2. b Induction of syncytia was observed in light microscope 5 and 7 days after infection. -, no syncytia; +, 10–20 syncytia per well; ++, syncytia covering 20–50% of the wells; +++, syncytia covering 50–90% of the wells; ++++, syncytia covering >90% of the wells
  3. c Virus production was measured six days after start of cocultures with human PBMC (hPBMC). Values are means of two independent infections in duplicate wells. Supernatant culture fluids were collected at day 7 and production of RT was analyzed. Supernatants were undiluted in the RT assay and therefore values above 1000 pg RT/ml cannot be separated. Cut-off detection level was 50 pg RT/ml