Figure 2

Progression of p30 expressing Jurkat T-cells through G2-M is delayed. A) Cell cycle distribution of mock or p30 transduced Jurkat T-cells at 6 h post release from hydroxyl urea block by propidium iodide staining followed by FACS analysis. Histogram was generated using ModFit^Rprogram (Verity Software House, Topsham, ME). Data represented is from one of the 4 independent trials B) A comparison of percentage of cells in the G2-M phase of the cell cycle of p30 and mock transduced Jurkat T-cells at indicated time points post hydroxyl urea release. Data represented is from a different trial than A. C) A comparison of percentage of cells in the S phase of cell cycle of p30 and mock transduced Jurkat T-cells at indicated time points post hydroxyl urea release. Data represented is from the same trial as B. D) Fold increase of cells in G2 phase of cell cycle in p30 Jurkat T-cells of 4 independent experiments was calculated by dividing the number of cells in G2 phase of cell cycle in p30 Jurkat T-cells over mock Jurkat T-cells. E) p30-expressing Jurkat T-cells or mock infected Jurkat T-cells were assayed for growth using a trypan blue exclusion assay. The p30-expressing Jurkat T-cell line growth curve differed from that of the mock infected Jurkat T-cell line (p-value 0.02 after adjusting for time in a quadratic model) due to an initial lag in the p30-expressing Jurkat T-cell growth rate compared to that of the mock infected Jurkat T-cells. By day 5, p30- expressing Jurkat T cell cultures had fewer total cell numbers compared to mock infected Jurkat T cell cultures (nonparametric Wilcoxon rank sum test, p-value 0.05). Bars indicate 95 % confidence interval.