Figure 4From: Recovery of fitness of a live attenuated simian immunodeficiency virus through compensation in both the coding and non-coding regions of the viral genomeRestoration of proteolytic Gag-processing by G49K, or by the E18G, G31K mutations. Viruses were purified by ultracentrifugation of clarified culture supernatants over a sucrose cushion at 48h after transfection. Western analysis of viral Pr55 Gag products were detected using MAb directed against p27-CA antigen.Back to article page