Figure 3
From: Nuclear Factor 90(NF90) targeted to TAR RNA inhibits transcriptional activation of HIV-1
NF90 inhibits Tat- trans-activation in concentration-dependent manner. The effect of NF90ctv on basal and Tat-mediated transactivation of HIV-1 LTR was assessed in both HeLa and Jurkat cells using CaCl2/Hepes transfection as follow. Duplicate 6 well- plates of HeLa cells received 2.5 ug of pHIV-β gal per 5 × 106 cells and increasing amounts (0.0 ug, 2.0 ug or 10.0 ug) of pCMV-NF90ctv. One set of plate (Transactivation received constant amounts (5.0 ug) of pSV2-Tat72. In each assay, 0.2 μg of pCMVCAT was cotransfected to monitor transfection efficiency. Following the transfection, cell extracts were prepared and standardized for total protein; colorimetric β-galactosidase and CAT assays were performed as before [37).