Figure 1
From: Nuclear Factor 90(NF90) targeted to TAR RNA inhibits transcriptional activation of HIV-1
Competition Analysis of RNA Binding Specificity. A: The structure of the wild type TAR RNA and the TAR mutants used in this assay are illustrated. B: 500 ng of protein from the TAR Fraction containing NF90 was incubated with 0.2 pmole radiolabeled TAR RNA (lanes 2, 6, 10, 14, 18). Competition for radiolabeled TAR RNA binding was done with increasing amounts of unlabeled TAR RNA (lanes 3, 5), TM12 RNA (lanes 7, 9), TM18 RNA (lanes 11–13), TM27 RNA (lanes 15–17), or TM12+TM27 RNAs (lanes 19–21). Samples were run on a 10%PAGE.