Figure 2
From: Expression pattern analysis of transcribed HERV sequences is complicated by ex vivorecombination
RECCO-based explanation of HERV-KX sequences as recombination products. Based on RECCO analysis, HERV-KX sequences can be explained as recombination products between transcripts from different HML-2 proviral loci. Three examples of HERV-KX sequences were multiply aligned with HML-2 proviral sequences, the transcripts of which served as template for recombination events. HERV-KX sequence "93" was generated by one recombination event involving two loci. HERV-KX sequence "B270" was generated by two recombination events involving three loci. HERV-KX sequence "94" was generated by two recombination events involving two different proviral templates. Probable recombination regions are indicated by medium grey background. Light and dark grey background indicates proviral templates. Localization of the RT-PCR amplicon and the 96 bp sequence (see text) within the HML-2 gag gene/provirus is depicted above the sequence comparisons.