Figure 9
From: Human Polycomb groupEED protein negatively affects HIV-1 assembly and release
Confocal fluorescence microscopy of 293T cells expressing EED alone (a) or WTNef alone (b), or co-expressing EED and various Nef proteins; (c),WTNef ;(d),LAT-Nef ;(e), NefG2A. The experimental protocol was as described in Fig. 8a. Left panels: anti-EED rabbit antibody and Alexa Fluor® 488-labeled goat anti-rabbit IgG ; middle panels: anti-Nef mAb and Alexa Fluor® 633-labeled goat anti-mouse IgG antibody; right panels: merged images. Note the absence of co-localization of EED and LAT-Nef, contrasting with the co-localization signals of EED and WTNef or NefG2A.