Figure 7
From: Human Polycomb groupEED protein negatively affects HIV-1 assembly and release
Effect of Nef mutants on EED. (a), Experimental protocol. HIV-Luc virions were produced by 293T cells transfected with pTracer-EED or pTracer-Emp, in the presence of various Nef proteins, WTNef, NefΔ57 mutant, or fusion constructs LAT-Nef or LATAA-Nef. Cell culture fluids were centrifuged and the amounts of virions in pellets quantified by genomic RNA levels. (b), Histogram of virion production in the presence of Nef without EED (grey bars) or EED with Nef (filled bars). (c), Ratio of virus yields in the presence versus the absence of EED, expressed as percentage. WTNef and LATAA-Nef showed the same EED antagonistic effect, whereas LAT-Nef and NefΔ57 mutants had a different phenotype.