Figure 6
From: Human Polycomb groupEED protein negatively affects HIV-1 assembly and release
Antagonistic effect of Nef on EED. (a), Experimental protocol. Pelleted HIV-Luc vector particles produced by 293T cells transfected with pTracer-EED (filled symbols) or pTracer-Emp (open symbols), with or without Nef protein, WTNef or NefG2A mutant, were assayed for (b) vector infectivity, determined by luciferase activity in recipient cells, or (c-f) further analyzed by sucrose-D2O gradient ultracentrifugation. Gradient fractions were analyzed for (c, d) CAp24 titer, and (e, f) genomic RNA level. WTNef protein counteracted the negative effect of EED, and restored the virus production to control levels.