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Figure 4 | Retrovirology

Figure 4

From: Human Polycomb groupEED protein negatively affects HIV-1 assembly and release

Figure 4

Gag protein expression in EED3/4-expressing cells. (a), Experimental protocol. (b, c), Dose-response analysis of EED3/4 effect on gag gene expression in cells cotransfected with pNL4-3Luc(R-E-) and pTracer-EED (or control pTracer-Emp), at varying plasmid inputs (0.25 to 1 μg/106 cells). (b), Autoradiogram of SDS-PAGE and immunoblots reacted with anti-Gag antibody and 35SLR-labeled complementary antibody. Band c, 20-kDa cellular protein used as internal control for protein load. (c), Histogram of the ratios of total Gag proteins synthesized in the presence of pTracer-EED versus pTracer-Emp. (d), Time-course analysis of pNL4-3Luc(R-E-)-mediated luciferase expression in 293T cells co-transfected with pTracer-EED (filled symbols) or control pTracer-Emp plasmid (open symbols) at constant plasmid input (1 μg/106 cells). A slight increase in Gag protein synthesis was detected in the presence of EED, at plasmid inputs higher than 0.5 μg. A similar positive effect of EED (2- to 5-fold) on luciferase levels was observed between 18 and 72 h posttransfection.

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