Figure 2
From: Human Polycomb groupEED protein negatively affects HIV-1 assembly and release
Antiviral effect of EED3/4 on incoming HIV-Luc virions. (a), Experimental protocol. (b), Time-course analysis of luciferase expression in 293T cells transfected with pTracer-EED, pTracer-EED394 (EED ST394AI mutant) or control pTracer-Emp at constant plasmid input (1 μg/106 cells), and infected with HIV-Luc vector. Luciferase activity, expressed as relative light units (RLU), was normalized to equal protein content. (c), Ratio of luciferase levels in cells expressing EED3/4 or EED394, versus control (pTracer-Emp). (d), Dose-response analysis of EED3/4 effect on luciferase expression.(e), Effect of the coexpression of WTNef or packaging-defective mutant NefG2A on EED antiviral activity. The discrete negative effect of EED on virus infectivity did not change when NefWT was provided in trans to virions in producer cells.