Figure 3

(A) A comparison of the morphologies of HeLa cells transduced with tax mutants. Morphology of Hela cells transduced with a lentivirus vector carrying, respectively, the wild-type, V19M, C23W, A108T, L159F, and L235F mutant tax alleles. The tax-transduced cells were selected in medium containing puromycin 1 μg/ml for 3 days. The puromycin-resistant colonies were photographed. The scale bar represents 20 μm. (B) Transactivation of a Tax-specific reporter HeLa cell line transduced with tax mutants. A HeLa cell line containing a stably integrated Tax reporter cassette, 18 × 21-DsRed, was transduced with a lentivirus vector, HR'CMV-SV-puro, harboring wild-type or each of the mutant tax alleles. The tax-transduced cells were then selected in medium as above. Puromycin-resistant colonies were visualized and photographed using an Olympus IX8 inverted fluorescence microscope. The scale bar represents 20 μm.