Figure 3

Analysis of serum response to various CN54 gp120s by ELISA and Western blot. A. Pooled mouse sera were assayed on highly purified CN54 gp120 in a reciprocal dilution series starting at 1:100 and binding detected with an anti-mouse conjugate. The immunogens were: (▲) – gp120_CN54⁺-Fc;(■) – OD_CN54⁺-Fc;(◆) – gp120_CN54⁺. B. Western blot analysis of each serum on gp120_CN54⁺ (track 1) OD_CN54⁺-Fc (track 2) and gp120_Bal (track3 – to assess cross clade reactivity). The panels were blotted with the sera raised to the proteins shown and were used at the dilutions indicated. Anti-Fc reactivity in the sera was blocked by pre-incubation with excess HCV E2-Fc (not described).