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Figure 1 | Retrovirology

Figure 1

From: Immunogenicity of the outer domain of a HIV-1 clade C gp120

Figure 1

Characterisation of HIV-1 gp120CN54 (V295N+A394T) expressed as a transmembrane bound protein in both Spodoptera frugiperda (Sf9) and Mimic cells (Invitrogen). A. Western blot of cell extracts (tracks 1 and 2) and supernatant (tracks 3 and 4) showing the increase in size associated with gp120 with modified glycans. Track 1 and 3 – Sf9 cells, Track 2 and 4 – Mimic cells. Note the presence of the unglycosylated apoprotein (arrowed) and glycosylation intermediates in the cell extracts which are absent in the supernatant. The gp120 present in the supernatant is due to pickup by budding baculovirus particles (see [53]). Markers to the left are in kilodaltons. B. Flow cytometry of permeablized or non-permeablized Sf9 and Mimic cells following staining with 2G12. Cells were permeablized by incubation in 0.1% Triton X-100 followed by fixing in 3% paraformaldehyde. Control cell profiles are shaded, infected cell profiles are unshaded.

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