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Figure 3 | Retrovirology

Figure 3

From: Antigen-presenting particle technology using inactivated surface-engineered viruses: induction of immune responses against infectious agents

Figure 3

Proliferation Index (PI) time course comparison in two donor (D and E) PBLs. Panel A: Non-engineered particles. For PHA (black-filled bars), the proliferation value in the presence of PHA (i.e. Donor-D, 6 hour timepoint = 4,000 relative fluorescent units) is divided by the value observed in untreated cultures (i.e. Donor-D, 6 hour timepoint = 2,000 relative fluorescent units); for HIV-1 (gray-filled bars), the proliferation value in the presence of non-engineered HIV-1 particles (i.e. Donor-D, 6 hour timepoint = 2,200 relative fluorescent units) is divided by the value observed in untreated cultures (i.e. Donor-D, 6 hour timepoint = 2,000 relative fluorescent units); for HSV-2 (horizontal hatched bars), the proliferation value in the presence of non-engineered HSV-2 particles (i.e. Donor-D, 6 hour timepoint = 2,000 relative fluorescent units) is divided by the value observed in untreated cultures (i.e. Donor-D, 6 hour timepoint = 2,000 relative fluorescent units); for Influenza A (PR8) (right-diagonal hatched bars), the proliferation value in the presence of non-engineered influenza-A particles (i.e. Donor-D, 6 hour timepoint = 26,000 relative fluorescent units) is divided by the value observed in untreated cultures (i.e. Donor-D, 6 hour timepoint = 2,000 relative fluorescent units); and for Influenza B (Russian) (left-diagonal hatched bars), the proliferation value in the presence of non-engineered influenza-B particles (i.e. Donor-D, 6 hour timepoint = 32,000 relative fluorescent units) is divided by the value observed in untreated cultures (i.e. Donor-D, 6 hour timepoint = 2,000 relative fluorescent units). Panel B: Surface-engineered influenza particles. For B7+antiCD3 surface-engineered influenza A (PR8) (right-diagonal hatched bars), the proliferation value in the presence of surface-engineered particles (i.e. Donor-D, 6 hour timepoint = 29,000 relative fluorescent units) is divided by the proliferation value observed with non-engineered influenza A particles (i.e. Donor-D, 6 hour timepoint = 26,000 relative fluorescent units); and for Influenza B (Russian) (left-diagonal hatched bars), the proliferation value in the presence of surface-engineered particles (i.e. Donor-D, 6 hour timepoint = 28,800 relative fluorescent units) is divided by the proliferation value observed with non-engineered influenza B particles (i.e. Donor-D, 6 hour timepoint = 32,000 relative fluorescent units). The time course shown in panels A and B for Donor-D is 4, 6, 10, 13, and 20 days; for Donor-E is 4, 10, 13, and 20 days. The remaining PI values are calculated in a similar fashion. Actual induced values can be calculated by multiplying the PI value by the "background" value. Particle preparations used in this figure were PEG-concentrated (200× for HIV; 25× for HSV; 40× for Influenza A/B) and inactivated to render them non-infectious.

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