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Figure 4 | Retrovirology

Figure 4

From: Efficient inhibition of HIV-1 expression by LNA modified antisense oligonucleotides and DNAzymes targeted to functionally selected binding sites

Figure 4

In vitro cleavage of HIV-1 RNA by DNA- and LNAzymes. One hundred nmol 5' end labeled leader RNA (+1–355) was incubated with 5 nmol, 100 nmol or 2 pmol DNAzymes or LNAzymes for the indicated time. The DNAzymes targeted to the PBSD and DIS regions cleaved primarily at the expected site, yielding a 5'-end labeled fragment of 205 and 261 nucleotides, respectively (product; panel A and C). The same bands were obtained using the LNAzyme (panel B and C). The experiment was made in duplicates yielding essentially the same result and the cleavage efficiencies indicated below each autoradiogram were calculated as (cleaved RNA/cleaved RNA and uncleaved RNA) × 100% averaged over both experiments.

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