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Figure 1 | Retrovirology

Figure 1

From: Efficient inhibition of HIV-1 expression by LNA modified antisense oligonucleotides and DNAzymes targeted to functionally selected binding sites

Figure 1

Oligonucleotides and their respective targets in the 5' end of the HIV-1 RNA genome. (A) Secondary structural model of the HIV-1 leader RNA. The stem-loops are named according to assigned function (see text for details) and the sequence is numbered from the 5' end of the RNA transcript. (B) The targets for the various oligonucleotide constructs. The annealing sites for the oligonucleotides are indicated by a solid line and the cleavage sites of the DNA/LNAzymes are marked by arrows. (C) Sequences of the antisense oligonucleotides and DNAzymes containing the 10–23 catalytic motif [10] named according to their target sites shown in panel B. The selected target sequences for the antisense constructs include sequences downstream of the primer binding site (PBSD), the dimerization initiation site (DIS), the splice donor site (SD) and the Gag initiation codon (AUG). The nucleotides that are substituted with LNA residues in the LNA antisense gap-mers and LNAzymes constructs are circled. The target sequences of the "10–23" DNAzymes are indicated with grey letters.

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