Effect of hnRNP E1/E2 Overexpression on the Subcellular Distribution of Viral RNAs. 293T cells were transfected with control (CMVpA) or mycE1/E2 expression plasmids as indicated, alongside pgTat and either an inactive form of Rev (M10) (A) or wild type Rev (B). Following harvest, nuclear and cytoplasmic fractions were prepared, RNA extracted and used in RNase protection assays. Protection products were resolved on denaturing PAGE gels. Identities of the various bands observed are indicated; unspliced, uncleaved (US/UC); unspliced, cleaved (US/C); spliced, uncleaved (S/UC); spliced, cleaved (S/C).