Skip to main content
Figure 4 | Retrovirology

Figure 4

From: Antagonistic interaction of HIV-1 Vpr with Hsf-mediated cellular heat shock response and Hsp16 in fission yeast (Schizosaccharomyces pombe)

Figure 4

Expression of hsp16 under exogenous nmt1 promoter rather than endogenous promoter suppresses Vpr-induced cell killing. (A) High temperature does not suppress Vpr-induced cell killing but expression of hsp16 through a foreign nmt1 promoter does. Plates in the top row are fission yeast cells streaked on thiamine-containing (vpr-off) EMM plates; bottom row plates are the same as the top plates except no thiamine (vpr-on) was added. Plates are shown after 3-5 days of incubation. (B) Induction of cellular heat shock responses does not affect the protein levels of the wild type Vpr (RE007) as indicated by the Western blot analysis. GI, gene induction.

Back to article page