Figure 7

Test of sequence-specificity of the inhibition by HIV-lhNef. (A) Trans-inhibition of HIV-1 production by HIV-lhNef and the RNAi-inducing shGag or shNef constructs. HEK293T cells were co-transfected with 100 ng HIV-1 and 100 ng pBluescript, 100 ng HIV-lhNef, shGag or shNef. CA-p24 production was measured in the culture supernatant at two days post-transfection. Error bars represent the standard deviation in two independent experiments. (B) Inhibition of the Luc-Nef reporter. HEK 239T cells were co-transfected with 100 ng of Luc-Nef and 100 ng HIV-1, HIV-lhNef, shGag or shNef. Error bars represent the standard deviation in two independent experiments.