Skip to main content
Figure 3 | Retrovirology

Figure 3

From: Trans-inhibition of HIV-1 by a long hairpin RNA expressed within the viral genome

Figure 3

Virus replication of HIV-lhNef and the escape variants in PBMC. Cells (5 × 106) were transfected with 10 μg of the proviral DNA constructs and CA-p24 production was measured in the culture supernatant at several days post-transfection for up to 4 weeks. Half of the culture medium was replaced with fresh complete RPMI medium with IL-2 every 4 days and freshly activated PBMC (2 × 106) were added at every 2nd addition. The experiment has been repeated five times for AS19 and two times for the other variants.

Back to article page