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Figure 1 | Retrovirology

Figure 1

From: Trans-inhibition of HIV-1 by a long hairpin RNA expressed within the viral genome

Figure 1

The HIV-lhNef RNA genome encodes an extended hairpin that is truncated by virus evolution. (A) The proviral DNA genome, highlighting the insertion of the antisense asNef fragment (original Nef coordinates +8544 to +8844). The primers tTA1 and CN1 used to amplify the inverted repeat region are indicated. The inverted repeat (thick arrows) results in the formation of a 300 bp hairpin structure in the RNA genome of HIV-lhNef (middle panel). This hairpin encompasses the polypurine tract (ppt). The truncated hairpin structure that emerged by virus evolution is shown in the lower panel. (B) HIV-lhNef escape variants were previously described in detail [28]. The name AS (antisense) reflects the number of basepairs in the remaining RNA hairpin. ΔG is the thermodynamic stability of the remaining perfectly basepaired stem segment. The replication column shows the replication capacity of the viruses.

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