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Figure 9 | Retrovirology

Figure 9

From: The HBZ-SP1 isoform of human T-cell leukemia virus type I represses JunB activity by sequestration into nuclear bodies

Figure 9

Targeting of endogenous JunB to HBZ-NBs in HeLa cells transfected with pEGFP-HBZ-SP1. (A) Nuclear localization of endogenous JunB in HeLa cells transfected with different mutants of the HBZ-SP1 protein. HeLa cells were transfected with pNLS-EGFP, pEGFP-HBZ-SP1, pEGFP-HBZΔAD. HeLa cells were arrested using a double thymidine block and restimulated with 10% FCS for the indicated times and the subnuclear localization of endogenous JunB was analyzed by immunofluorescence microscopy. JunB was detected using a mouse anti-JunB antibody and goat anti-mouse IgG antibody coupled to Texas Red. (B) Colocalization of endogenous JunB with EGFP-HBZ-SP1 and EGFP-HBZΔAD in transfected HeLa cells arrested in the cell cycle. The shown data correspond to t = 0 of Fig. 9A and are representative of the experiments obtained at different times. Analysis of the green, red, and merged fluorescent signals was performed by fluorescence microscopy. The white bars correspond to a scale of 10 μm.

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