Figure 4

Stimulation of HBZ-NBs formation by JunB. COS cells were transiently transfected with EGFP (a), HBZ bZIP fused to EGFP (b), or JunB (c), cultivated on glass slides, fixed, and then were analyzed by fluorescence microscopy. JunB was detected using a mouse anti-JunB antibody and goat anti-mouse IgG antibody coupled to Texas Red. JunB was also cotransfected into COS cells with either EGFP-HBZ bZIP (d-f), or EGFP-HBZ-SP1 (g-i), or EGFP (j-l). Analysis of the green (a, b, d, g, and j), red (c, e, h, and k) and merged (f, i, and l) fluorescent signals was performed by fluorescence microscopy. The white bars correspond to a scale of 10 μm.