Figure 2

Molecular structures and nuclear localizations of HBZ-SP1 and its deletion mutants fused to EGFP. HBZ is composed of an N-terminal activation domain (AD), two basic regions (BR1 and BR2) involved in its nuclear transport, a transcriptional modulatory domain (MD), and a C-terminal bZIP domain. The HBZ-SP1 protein and its mutants fused to EGFP were transiently transfected into COS cells (column EGFP). Cells were cultivated on glass slides, fixed and treated with Vectashield containing DAPI for direct observation by fluorescence microscopy. Transfected COS cells were also labelled with a mouse anti-nucleolin antibody (column anti-nucleolin) and detected using goat anti-mouse IgG antibody coupled to Texas Red. Analysis of the green, red, and merged fluorescent (column merge) signals was performed by fluorescence microscopy. The white bars correspond to a scale of 10 μm.