Figure 6
Analysis of HXB2-Met(i) with A to G mutation in PBS. Panel A. HXB2-Met(i) with A to G mutation. The PBS of HXB2-Met(i) with the ATG codon as boxed. A new mutant, HXB2-Met(i)AG was constructed in which the adenine was changed to guanine to eliminate the ATG (boxed). Panel B. Production of p24 following transfection. Proviral genome HXB2-Met(i)AG was transfected into 293T cells and the amount of virus produced was determined using the p24 antigen ELISA assay. For comparison, the p24 values for HXB2-Met(e), HXB2-Met(i) and HXB2-WT are presented. Error bars ± standard deviation. Panel C. Production of infectious virus following transfection of proviral genomes into 293T cells. The amount of infectious virus is determined by the JC53-BL assay was determined for viruses derived from transfection of HXB2-Met(i)AG. For comparison, the amounts of infectious virus from HXB2-Met(e), HXB2-Met(i) and HXB2-WT are also presented. Error bars ± standard deviation.Panel D. Replication of HXB2-Met(i) with A to G mutation in SupT1 cells. The replication of HXB2-Met(i)AG was analyzed in SupT1 cells. The amount of virus produced was determined by p24 antigen capture assay. Data is representative from two independent experiments. The samples are as marked in the figure.