Figure 1

tRNA and HIV-1 proviruses. Panel A. tRNA^Met(e) and tRNA^Met(i). HeLa cell tRNA^Met(e) and tRNA^Met(i). The nucleotides shown in large boldface type in the tRNA are complementary to the PBS of the viral RNA genome. Diagram of tRNA^Met as described by Harada et al [49]. Panel B. Genomes with PBS complementary to tRNA^Met(e) or tRNA^Met(i). The 5' region of the HIV-1 RNA genome is expanded to depict the locations of sequences having complementarity with the 3' 18 nucleotides of the tRNA (bolded). The wild-type PBS (nucleotides 183 to 200) in HXB2 was replaced with the PBS complementary to the 3'-terminal 18 nucleotides of tRNA^Met(e) [HXB2-Met(e)] or tRNA^Met(i) [HXB2-Met(i)].