Figure 4

Non-denaturing Northern analysis of SIV RNA. Non-denaturing analysis of intra-virion RNA from transient transfections of mutant clones. Viral genomic RNA was isolated from virus particles after transfection of COS-7 cells with wild type or mutant plasmids. The relative mobility of dimers (D) and monomers (M) in 0.90% agarose are indicated. The plus (+) denotes the addition of RNase to preparations prior to electrophoresis. A. Shows non-denaturing RNA preparations from deletion mutants encompassing the region from nt +426 - +465. B. Shows non-denaturing RNA preparations from mutants encompassing the nt region +473 - +480. C. RNA preparations from mutants encompassing the region directly adjacent to the PBS, i.e. SD1, SD2, and SD3 deleted the of nucleotide regions +322-344, +398-418, and +345-397 respectively [13]. The adjacent panel shows thermal denaturing analysis of the SD1 mutant (Δ+322 - +344), comprising a 23-nucleotide deletion upstream of SL1 in comparison to dimer extracted from the wild-type virions.