Skip to main content
Figure 3 | Retrovirology

Figure 3

From: Impaired RNA incorporation and dimerization in live attenuated leader-variants of SIVmac239

Figure 3

Both genomic RNA packaging efficiency and specificity are compromised in SIV mac239 by mutations within the leader region. A. Analysis of viral RNA packaging was conducted in triplicate in COS-7 cells. Shown are results from purified viral RNA preparations that were normalized on the basis of p27-CA ELISA. RNA packaging of various mutant constructs as a percent average of WT virus. The bars of the first lane for each sample represent total incorporated viral RNA. The second bar for each lane represents incorporated full-length viral RNA. B. Shown at the bottom is percent specific incorporation as a ratio of that obtained with the wild type full-length genome.

Back to article page